Composite

Part:BBa_K4762005:Design

Designed by: Xuan Hang   Group: iGEM23_NWU-CHINA-A   (2023-10-11)


T7_Promoter-RBS-CAT-T7_Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1260
    Illegal PstI site found at 1093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1260
    Illegal NheI site found at 1075
    Illegal PstI site found at 1093
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1260
    Illegal BamHI site found at 57
    Illegal BamHI site found at 1510
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1260
    Illegal PstI site found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1260
    Illegal PstI site found at 1093
    Illegal AgeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon optimization is carried out


Source

It comes from Escherichia coli O157:H7

References